... Table of contents Table of contents ii Summary v List of abbreviations vii List of tables ix List of figures ix Chapter Overview of research ... mM PDCA and 10 μM HDZ showed blood vessel infiltration comparable to VEGF delivery, and at this point, served as a proofof-concept that local delivery of PHi can aid angiogenesis vi List of abbreviations ... prophylactic setting of small skin wounds [61] 2.3 Understanding the last mile of the fibrotic pathway Irrespective of upstream events that trigger and entertain fibrosis, the final product of cellular...
... thanks to the staff of Highlander’s Coffee cafe for the wonderful coffee and great environment for us to brainstorm our ideas and work Everyone in NUS Nanobiomechanics Laboratory and NUS Regenerative ... period of days Immunofluorescent staining of hMSCs showed an upregulation of synaptophysin, indicating the establishment of synapse and electrical communication between cells Upregulation of connexin ... Quantum-dot labeling of collagen in PEC fibers and detection of collagen in (A) Sub-micron fibrils, (B) and (C) main fiber and (D) fiber bead region 70 Figure 4.11: Swelling studies of PEC and Polypyrrole...
... casting and solvent-free method of hot roll-milling • The exploration of the use of lasers to modify the surface of PCL films and understanding the mechanisms behind it, and the effect of the ... and elongation (λ) of all types of PCL films (Page 52) Table 4.1 Thermal and optical properties of PCL (Page 67) xi List of Figures Fig 2.1 Comparison of moduli of elasticity of biomaterials [15] ... the degree of Doctor of Philosophy in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Associate Professor Hong...
... usually show only γ-γ dimers and loss of α and γ bands as well as a-a multimers (Fig 2) Determine the density of the FMB by layering an aliquot of it onto a sucrose solution of known density After ... between HA and the coupling reagent is chosen as a function of the degree of substitution of the ester For a total esterification (>90% of carboxyl groups esterified), use an excess of 25–30% of benzyl ... micrograph of a porous chitosan scaffold Hydrated scaffolds are soft, spongy, and highly extensible The tensile elastic modulus of hydrated samples is in the range of 100–500 kPa (16), and equilibrium...
... with a step size of 0.02° and a step time of s The powder samples were placed on a flat silicon zero background sample holder The particle size distribution of a regular suspension and nanosuspension ... chromatographic tandem mass spectrometric (LC/MS/MS) The stability of the DCU formulations (both regular suspension and nanosuspension) was assessed, and no issue was found No particle size, potency, and ... dosing of 30 mg/kg of DCU nanosuspension twice a day (b.i.d.) is needed to provide continuous coverage of the plasma concentrations of 0.2 μM (1 × cellular IC50 corrected for free fraction) and...
... predisposes the patient to tooth mobility and loss, affects at least 15% of adults over the age of 30 years of age [51] In the US, the economic cost of treating and preventing periodontal disease was ... delivery of the DNA of interest to the pulpal/periodontal tissue, or the isolation of a small amount of gingival tissue from the patient, transduction/transfection of the DNA at chairside, and reimplantion ... similarity to the human periodontium in terms of structure, plaque flora, and inflammatory infiltrate), and beagle dogs (which have a different microflora and much faster bone turnover rate compared...
... cultivated and fabricated tissues may be used for studying the primary mixing of cells, and the patterns of cell differentiation and growth within growing spheres in order to improve the outcome of microsphere ... development of high-throughput systems, and allow manipulation of individual spheres It seems worthwhile elaborating new bioreactor technologies and culture techniques to improve the ex vivo growth of ... systems can be generated ex vivo without the use ofscaffolds Co-cultures of osteoblasts and endothelial cells for example resulted in the formation of a bi-cellular micromass tissue renouncing any...
... receptor x List of Tables Table 1.1 Summary of the advantages and limitations of the most commonly used vascularization strategies Table 2.1 A selected list of prolyl hydroxylase inhibitors and their ... tissue availability and donor site morbidity, while allogeneic and xenogeneic transplants have problems of immunogenicity and risks of disease transmission [4, 5] In addition, the demand for allogeneic ... stabilized and is thus not secreted, resulting in decreased collagen deposition Based on the understanding of collagen P4H’s structure, stereochemistry, and mechanism of action, a variety of small...
... of typical steps like: • • • • • Development of a control process model Design and programming of a first simple control Calibration of sensors and actuators Conception of performance tests and ... for demonstrations of a forklift handling device and optical line following system A rock and sand landscape similar to Mars, including facilities for a tether link to power and control rovers ... WWW-server that interfaces with clients and handles over the Internet the transfer of sensor data from the operator workplace and commands to the rover The WWW-server and the operator workplace exchange...
... 2) and every months after the end of VMAT Statistical analysis OS and PFS were calculated using the Kaplan Meier method [17] Recorded events were death (all causes of Page of death included) and ... Hospital/University of Geneva, CH-1211 Geneva 14, Switzerland 2University of Geneva, CH-1211 Geneva, Switzerland Authors’ contributions DCW was responsible for the primary concept and the design of the study; ... 31.2 and 23.8 ± 30.5 before and months after VMAT, respectively (p = 0.48; Table 3) MMSE, KPS and self-assessed QoL were also compared for the time points before and months after the start of VMAT...
... special attention to the fabricationof biologically active scaffoldsand matrices capable of fulfilling natural properties of the airway ECM, for example, by maintaining and slowly releasing factors ... cell-binding site for ligation of cell-surface integrin receptors and substrates for MMPs, proteases implicated in wound healing and tissue regeneration [51,52] andfabricationofscaffolds with immobilized ... injuries and the regeneration of the epithelial structure involve stem and progenitor cells The mechanism of the regeneration of the airway epithelium was widely studied in rodent models of lung...
... PM, Bieniasz P, Hahn BH, Hatziioannou T, Kirchhoff F: Tetherin-driven adaptation of Vpu and Nef function and the evolution of pandemic and nonpandemic HIV-1 strains Cell Host Microbe 2009, 6:409-421 ... Page of 13 Figure Mutation of S52A impairs Vpu-mediated degradation of CD4 and tetherin (A) Western blot analysis of Vpu expression in lysates of transfected 293T cells (B) FACS analysis of CD4 ... http://www.retrovirology.com/content/7/1/1 Page of 13 Figure Modulation of tetherin and CD4 in primary T-cells and macrophages by Vpu (A) FACS analysis of CD4 and tetherin modulation in infected PBL...
... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... providing the bulk of the tensile yield strength and plays an important in the storage of various ions The mineral salts of bone comprises of largely of calcium and phosphorus (Downey and Siegel 2006) ... first days of culture Figure 7-3 (A, C) Experimental work-flow of cellular-treatment before and during Matrigel assay and (B, D) quantification of quantification of branch points of vessel networks...
... Formation of CLS not follow scaffold fiber orientation LIST OF TABLES Table Summary of the in vitro and in vivo angiogenic and anti-fibrotic effects of PDCA, CPX, HDZ and VEGF xi LIST OF ABBREVIATIONS ... of CPX and VEGF treated admixed co-culture of fibroblasts and HUVEC in PLLA scaffolds 20x magnification Figure 38 Confocal microscopy (z-stack) of µM CPX treated admixed co-cultures in PLLA scaffolds ... cytotoxicity of HDZ, CPX and PDCA in monocultures or mixed cultures of IMR90 fibroblasts and HUVECs Figure 32 PHi inhibition of fibroblast collagen production in vitro SDS-PAGE and desitometric...
... layer of the periosteum is a 3D layer comprising of highly fibrous tissues and vascular network, hence mimicry of this layer would require 3D integration of cells and scaffold Modifications of the ... synthetic polymers is often directed at the creation of specialized and more biologically relevant materials Composite scaffoldsof natural-synthetic materials offer desired mechanical and manufacturing ... pre-determined period of time resulted in tough yet flexible nanofiber sheets (Fig 4B, C) that could be handled for downstream applications Morphology and size distribution of PCL and PCL/Col nanofibers were...
... degree of Doctor of Philosophy in the Graduate Program in Bioengineering at the National University of Singapore under the supervision of Professor Lee Chuen Neng, Professor Teoh Swee Hin and Dr ... years and has saved many lives, while having improved the quality of lives of thousands of other patients Paradoxically, the advent of reliable transplantation techniques created the issue of organ ... formation of a confluent EC layer in vivo and evidence of tissue ingrowth and vitalisation of the construct (Greisler 1985; Greisler 1987) However, the extent of tissue formation was insufficient, and...
... maintenance and substitution of damaged tissues and organs using principles of biology and engineering (Langer and Vacanti, 1993) BTE consists of an interplay of scaffold technology, growth factors and ... B: PCL-TCP scaffolds loaded with 100ng/ml BMP-2 Group C: PCL-TCP scaffolds loaded with 300ng/ml of heparin Group D: PCL-TCP scaffolds loaded with 300ng/ml of heparin and 100ng/ml of BMP2) Figure ... Amount of BMP-2 release at various time points (Group B: PCLTCP scaffolds loaded with 100ng/ml BMP-2 Group D: PCL-TCP scaffolds loaded with 300ng/ml of heparin and 100ng/ml of BMP2) Group A and...
... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... Table 4.1 Mw, Mn, and PDI of native, NaOH-treated, and lipase-treated PCL-TCP Scaffolds in vitro 66 Table 4.2 Mw, Mn, and PDI of native, NaOH-treated, and lipase-treated PCL-TCP Scaffolds in vivo ... diagram of part and part study Figure 2.1 Composition of bone Figure 2.2 The assembly of collagen fibrils and fibers and bone mineral crystals Figure 2.3 Microscopic structure of cortical and cancellous...
... Engineering GRAND CHALLENGE 2: OPTIMIZING STRUCTURAL SYSTEMS To understand the advantages and limitations of traditional, newer and emerging materials in terms of safety, durability and economy; and to ... understand the time-restraints, durability and economy of traditional bridge systems and their construction methods, and the possibilities and limitations of newer accelerated methods, and to ... Implementation and further development of design considerations for hardening of existing structures and rapid recovery after disasters (natural and manmade) Implementation ofand further development of...